恺佧生物科技（上海）有限公司

表达区间及表达系统（Source）	Recombinant Human Sonic Hedgehog (Shh) Protein is expressed from E.coli without tag. It contains Cys24-Gly197 [Accession \| Q15465].
分子量大小（Molecular Weight）	The protein has a predicted MW of 19.79 kDa kDa. The protein migrates to 23-25 kDa based on Bis-Tris PAGE result.
纯度（Purity）	> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素（Endotoxin）	Less than 0.05EU per μg by the LAL method.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储（Storage）	-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

表达区间及表达系统（Source）

Recombinant Human Sonic Hedgehog (Shh) Protein is expressed from E.coli without tag.
It contains Cys24-Gly197 [Accession | Q15465].

分子量大小（Molecular Weight）

The protein has a predicted MW of 19.79 kDa kDa. The protein migrates to 23-25 kDa based on Bis-Tris PAGE result.

纯度（Purity）

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素（Endotoxin）

Less than 0.05EU per μg by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储（Storage）

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.